Mimi mouse song

Added: Aliya Victor - Date: 19.01.2022 07:13 - Views: 31370 - Clicks: 3796

Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions and can augment or diversify existing functions. Here, we report a gene duplication event that yielded another outcome--the generation of antagonistic functions. Using loss-of-function approaches in vitro and in vivo, we discovered that UPF3A acts primarily as a potent NMD inhibitor that stabilizes hundreds of transcripts.

Evidence suggests that UPF3A acquired repressor activity through simple impairment of a critical domain, a rapid mechanism that may have been widely used in evolution. Our support a model in which UPF3A serves as a molecular rheostat that directs developmental events. Please enable it to take advantage of the complete set of features! Clipboard, Search History, and several other advanced features are temporarily unavailable.

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Mimi mouse song

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Mimi mouse song

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Mimi mouse song

RSS Link Copy. Full text links Cite Display options Display options. Abstract Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions and can augment or diversify existing functions.

Figures Figure 1. Figure 1. P19 cells were transfected with the constructs described in panel A, followed by ActD treatment for the indicated times. P19 cells were transfected with the constructs described in panel A and analyzed by the NMD reporter described in the Materials and Methods. Graphs are represented as mean and standard error SEM of replicates. Figure 2. UPF3A-stabilized and -destabilized transcripts are blue and green, respectively. Transcripts not exhibiting altered stability are gray. Darker shades of blue and green convey progressively increasing regulation. Evolution and Functional Analysis of….

Figure 3. A Two copies of upf3 appear to have emerged at the dawn of vertebrates.

Mimi mouse song

See Figure S3A for a phylogenetic tree showing the evolution of these paralogs. Figure 4. These UPF3 mutants were ly shown to be expressed at levels similar to that of the corresponding wild-type UPF3 proteins Kunz et al. Experiments were performed as in Figure 3B with the constructs shown in Figure 4B.

F Left: Northern blot analysis of HeLa cells co-transfected with the indicated expression vectors and siRNAs, along with both the reporters described in panel E. Upf3a is Required for Early…. Figure 5.

Mimi mouse song

A Upf3a conditional knockout scheme and location of primers used for the detection of the floxed and knockout alleles at the Upf3a locus. B Genomic PCR analysis of tails from mice with the indicated genotypes. The data show successful insertion of the targeted allele harboring loxP sites.

The embryos were manually flushed out of fallopian tubes of superovulated mice and the distribution of genotypes in different morphological groups is shown. DAPI staining blue shows the position of nuclei. Figure 6. Panel A shows adult mouse tissues and Panel B shows germ cell subsets. Pre-immune IgG serves as a negative control. L, leptotene spermatocyte; P, pachytene spermatocyte; S, spermatid. Most seminiferous tubules in the mutant had delayed spermatogenesis at P28, as evidenced by the presence of pachytene spermatocytes P and round spermatids RS near the lumen.

Wild-type tubules typically have elongated spermatids ES at the lumen. Green arrows points to vacuoles, which were commonly present in mutant testes. Figure 7. Population A, leptotene spermatocytes; population B, zygotene-pachytene-diplotene spermatocytes; and population C, round and elongated spermatids. The 4N spermatocytes were purified as in panel C. See this image and copyright information in PMC. Gerhardt K. Biol Reprod. Epub May PMID: No abstract available. Chan WK, et al. Nat Struct Mol Biol. Epub Jun 7. PMID: Nguyen LS, et al. Mol Psychiatry. Mimi mouse song Dec RNA decay, evolution, and the testis.

Jones SH, Wilkinson M. Jones SH, et al. RNA Biol. Epub Dec 2. The UPF3B gene, implicated in intellectual disability, autism, ADHD and childhood onset schizophrenia regulates neural progenitor cell behaviour and neuronal outgrowth. Jolly LA, et al. Hum Mol Genet.

Mimi mouse song

Epub Jul 2. Deka B, et al. Epub Oct PMID: Review. See all similar articles. Kitamura Y, et al. Sci Rep. Nonsense-mediated RNA decay and its bipolar function in cancer. Nogueira G, et al. Mol Cancer. Regulation of nonsense-mediated mRNA decay in neural development and disease.

Lee PJ, et al.

Mimi mouse song

J Mol Cell Biol. Ghiasi SM, et al. Front Endocrinol Lausanne. Man Z, et al. Front Oncol. See all "Cited by" articles. Publication types Research Support, N. Research Support, Non-U. Gov't Actions. MeSH terms Animals Actions. Cell Line, Tumor Actions. Evolution, Molecular Actions. Gametogenesis Actions. HeLa Cells Actions. Humans Actions. Mice Actions. UPF 3A protein, mouse Actions.

UPF3B protein, mouse Actions. Show all 9 grants. Copy Download.

Mimi mouse song

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The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay